RNA isolation / purification

Get tips on using Quick-DNA™ Miniprep Kit to perform DNA isolation / purification Tissue - adipose

Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Tissue - lung

Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Tissue - liver

Get tips on using Quick-DNA™ FFPE Kit to perform DNA isolation / purification Tissue - kidney

Get tips on using EZ1 Virus Mini Kit v2.0 (48) to perform DNA isolation / purification Viral

Get tips on using QIAamp BiOstic Bacteremia DNA Kit (50) to perform DNA isolation / purification Fish

Get tips on using QIAamp MinElute Virus Spin Kit (50) to perform DNA isolation / purification Viral

Get tips on using illustra GFX PCR DNA and Gel Band Purification Kits to perform

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.