siRNA / RNAi /miRNA transfection Human Cells Jurkat cells

Get tips on using - to perform siRNA / miRNA gene silencing Rat - IEC-6

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - Human CD14+ cells

Get tips on using Quick-RNA Microprep Kit to perform RNA isolation / purification Cells - primary human aortic endothelial cells

Get tips on using Quick-RNA™ MiniPrep Plus to perform RNA isolation / purification Cells - primary human epithelial cells

Get tips on using NucleoSpin® RNA/Protein to perform Protein isolation Mammalian cells - Human eutopic endometrial stromal cells

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

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Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical vein smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical artery smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human renal proximal tubular epithelial cells