rna-isolation-purification-tissue-mouse-spinal-cord

Get tips on using Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System to perform RNA amplification & labeling Mammalian - RNA amplification and Labeling Brain tissue from rhesus monkey Biotin

Get tips on using Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System to perform Microarray RNA amplification & Labeling - Rhesus monkey brain tissue Biotin

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Mouse - RAW264.7

Get tips on using DNA-spin™ Plasmid DNA Purification Kit to perform Plasmid Isolation Enterobacteriaceae

Get tips on using PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit to perform DNA isolation / purification Yeast - Cryptococcus neoformans

Get tips on using Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System to perform Microarray Rhesus monkey - Brain tissue Target preparation (RNA amplification + labeling)

Get tips on using MagMAX™ Total Nucleic Acid Isolation Kit to perform DNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Get tips on using Ovation® RNA-Seq System V2 to perform RNA sequencing Mouse - C2C12

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Mouse - Microglia

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.