siRNA / RNAi /miRNA transfection Human Cells HESC

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human skeletal muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human marrow stromal cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human bronchial epithelial cells

Get tips on using AquaRNA Kit to perform RNA isolation / purification Cells - primary human mesenchymal stem cells

Get tips on using RosetteSep™ Human Monocyte Enrichment Cocktail to perform Cell Isolation Monocyte

Get tips on using EasySep™ Human Monocyte Enrichment Kit to perform Cell Isolation Monocyte

Get tips on using EasySep™ Human Monocyte Isolation Kit to perform Cell Isolation Monocyte

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human mononuclear cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human endothelial cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.