rna-sequencing-mouse-3t3-l1

Get tips on using EpiTect ChIP qPCR Assays to perform ChIP Mouse - NIH3T3

Get tips on using HSP70 ELISA Kit (ab133060) to perform ELISA Mouse - HSP70

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.

Get tips on using Rn_Tlr3_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - Neuro 2a TLR3

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay mouse - C2C12

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay mouse - L929

Get tips on using Anti-CCSP - Rabbit to perform Flow cytometry Anti-bodies Mouse - CCSP

Get tips on using Anti-ZBTB16 antibody produced in rabbit to perform Immunohistochemistry Mouse - PLZF

Get tips on using Anti-Glucagon antibody produced in rabbit to perform Immunohistochemistry Mouse - Glucagon

Get tips on using Active BDNF (Human, Rat) ELISA Kit to perform ELISA Mouse - GDNF