crispr-mouse-deletion-raw-264-7-dcstamp

Get tips on using Tak1 Antibody (C-9): sc-7967 to perform Western blotting Tak1

Get tips on using VEGF Antibody (C-1): sc-7269 to perform Western blotting VEGF

Get tips on using Smad1 Antibody (A-4): sc-7965 to perform Western blotting Smad1

Get tips on using Smad4 Antibody (B-8): sc-7966 to perform Western blotting Smad4

Get tips on using Integrin β1 Antibody (102DF5): sc-73610 to perform Western blotting ITGB1

Get tips on using Anti-ATG5 (C-terminal) antibody produced in rabbit to perform Autophagy assay cell type - Rat spinal cord tissue

Get tips on using Mucin 1 Antibody (VU4H5): sc-7313 to perform Immunohistochemistry Human - Muc-1

Get tips on using IKKα Antibody (B-8): sc-7606 to perform Western blotting IKK Alpha

Get tips on using caveolin-1 Antibody (7C8): sc-53564 to perform Western blotting Caveolin-1

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.