siRNA / RNAi /miRNA transfection Human Cal 27 cells

Get tips on using EGFR (Full-length) Human ELISA Kit to perform ELISA Human - EGFR

Get tips on using Human Decorin ELISA Kit (DCN) (ab99998) to perform ELISA Human - Decorin

Get tips on using Human BDNF PicoKine™ ELISA Kit to perform ELISA Human - BDNF

Get tips on using Adiponectin Human ELISA, High Sensitivity (Sandwich) to perform ELISA Human - Adiponectin

Get tips on using VWF (Human) ELISA Kit to perform ELISA Mouse - vWF-A2

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Human TNF alpha ELISA Kit (ab181421) to perform ELISA Human - TNF-alpha

Get tips on using Human TNF-alpha Quantikine ELISA Kit to perform ELISA Human - TNF-alpha

Get tips on using Human PDGF BB ELISA Kit (ab100624) to perform ELISA Human - PDGF-BB

Get tips on using Human HO 1 ELISA Kit (ab133064) to perform ELISA Human - HO-1