ChIP H3K36Me3 Bovine Rabbit

Get tips on using EZ-ChIP™ to perform ChIP Human - PANC-1

Get tips on using Polyclonal Rabbit Anti-Human Myeloperoxidase (Dako Omnis) to perform Immunohistochemistry Mouse - MPO

Get tips on using Anti-UCP-1 antibody produced in rabbit to perform Immunohistochemistry Mouse - UCP1

Get tips on using PCNA (D3H8P) XP® Rabbit mAb #13110 to perform Immunohistochemistry Mouse - PCNA

Get tips on using Notch1 (D1E11) XP® Rabbit mAb #3608 to perform Immunohistochemistry Mouse - Notch1

Get tips on using Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb #4147 to perform Immunohistochemistry Mouse - Notch1

Get tips on using VEGF Receptor 2 (55B11) Rabbit mAb #2479 to perform Western blotting VEGF

Get tips on using Anti-Estrogen Receptor (ER) (SP1), Rabbit Monoclonal Primary Antibody to perform Immunohistochemistry Estrogen receptor (ER) - Rabbit Human -NA-

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Get tips on using ICAM-1 Recombinant Rabbit Monoclonal Antibody (9H21L19) to perform Western blotting ICAM-1