Site Directed Mutagenesis (SDM) Rat Point mutation

Get tips on using ON-TARGETplus Rat Tp53 (24842) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - C6 (rat glioma) p53

Get tips on using Rat VEGF ELISA Kit (ab100786) to perform ELISA Rat - VEGF

Get tips on using Rat VEGF Quantikine ELISA Kit to perform ELISA Rat - VEGF

Get tips on using Rat prolactin,PRL ELISA Kit to perform ELISA Rat - PRL

Get tips on using Rat GDNF ELISA Kit (ab213901) to perform ELISA Rat - GDNF

Get tips on using Rat Fibronectin ELISA Kit (ab108850) to perform ELISA Rat - Fibronectin

Get tips on using Mouse Rat Estradiol ELISA Kit to perform ELISA Rat - Estradiol

Get tips on using Rat CX3CL1/Fractalkine DuoSet ELISA to perform ELISA Rat - CX3CL1

Get tips on using Rat Adiponectin ELISA Kit (ab108784) to perform ELISA Rat - Adiponectin

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.