shRNA gene silencing Human Neuroblastoma cells (SH-SY5Y)

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The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

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Get tips on using JetPrime to perform DNA transfection Mammalian cells - Primary cells Human lung fibroblasts (HLF)

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human airway epithelial cells