Microarray Gene expression arrays Mouse liver tissue

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Get tips on using Monoclonal Mouse Anti-Villin (Autostainer Link 48) Clone 1D2 C3p to perform DNA Damage Assay U266 -

Products NSJ Bioreagents Monoclonal Mouse Anti-Villin (Autostainer Link 48) Clone 1D2 C3p

Get tips on using Purified Mouse Anti-E-Cadherin Clone 36/E-Cadherin (RUO) to perform Immunohistochemistry Human - E-Cadherin

Products BD Biosciences Purified Mouse Anti-E-Cadherin Clone 36/E-Cadherin (RUO)

Get tips on using Monoclonal Mouse Anti-Thyroid Transcription Factor (Concentrate) Clone 8G7G3/1 to perform Immunohistochemistry Human - TTF-1

Products Agilent Technologies Monoclonal Mouse Anti-Thyroid Transcription Factor (Concentrate) Clone 8G7G3/1

Get tips on using Purified Mouse Anti-β-Catenin Clone 14/Beta-Catenin (RUO) to perform Immunohistochemistry Human - β-catenin

Products BD Biosciences Purified Mouse Anti-β-Catenin Clone 14/Beta-Catenin (RUO)

RNA RNA isolation / purification Cells primary mouse morula cells

Get tips on using Mouse Monoclonal Antibody to Lamin A/C Cat# MCA-4C4 to perform Western blotting Lamin A/C

Products EnCor Biotechnology Inc. Mouse Monoclonal Antibody to Lamin A/C Cat# MCA-4C4

Get tips on using Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit to perform ELISA Rat - TGF-beta 1

Products R&D Systems Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Discussions siRNA/RNAi/miRNA transfection human

RNA siRNA / miRNA gene silencing Rat PC12 chorein

RNA siRNA / miRNA gene silencing Rat PC12 SOD2

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