Get tips on using 2x Laemmli Sample Buffer to perform Protein isolation Bacteria - Vibrio alginolyticus
Get tips on using TRI Reagent™ Solution to perform Protein isolation Bacteria - Vibrio cholerae
Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.
Get tips on using Midnight RT PCR Expansion to perform PCR Conventional / Qualitative PCR - Viral DNA
Get tips on using EMBacY#5 to perform Protein Expression Eukaryotic cells - Hi5 Soluble G protein (Hendra Virus)
Get tips on using BHI - Brain Heart Infusion to perform Bacterial cell culture media Vibro parahaemolyticus
Get tips on using BHI - Brain Heart Infusion to perform Bacterial cell culture media Vibrio cholera
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro cholerae
Get tips on using RNeasy PowerMicrobiome Kit (50) to perform RNA isolation / purification Viral - SARS-CoV-2
Get tips on using pSA-HVif-FabV to perform Protein Expression Prokaryotic cells - E. coli HIV-1 vif
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