An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human airway epithelial cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human peripheral blood cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human mammary mesenchymal cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human mammary epithelial cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human blood endothelial cell
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary bovine monocyte derived macrophages
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human skeletal muscle cells
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment