Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using Purified Mouse Anti-Human CD135 to perform Flow cytometry Anti-bodies Human - CD135
Get tips on using PE Mouse Anti-Human CD135 to perform Flow cytometry Anti-bodies Human - CD135
Get tips on using PE Mouse Anti-Human CD122 to perform Flow cytometry Anti-bodies Human - CD122
Get tips on using PE Mouse Anti-Human CD116 to perform Flow cytometry Anti-bodies Human - CD116
Get tips on using PE Mouse Anti-Human CD114 to perform Flow cytometry Anti-bodies Human - CD114
Get tips on using PE Mouse anti-Human CD96 to perform Flow cytometry Anti-bodies Human - CD96
Get tips on using Purified Mouse Anti-Human CD61 to perform Flow cytometry Anti-bodies Human - CD61
Get tips on using PE Mouse anti-Human CD52 to perform Flow cytometry Anti-bodies Human - CD52
Get tips on using PE anti-human CD52 Antibody to perform Flow cytometry Anti-bodies Human - CD52
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