RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Rat Colon

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Rat Cerebellum

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Rat Adipose

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Hippocampus

Get tips on using RNAsimple Total RNA Kit to perform RNA isolation / purification Tissue - Rat Placenta

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.