Site Directed Mutagenesis (SDM) Human Deletion MCF-7

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Keratinocyte SFM (1X) to perform 3D Cell Culture Media Human prostate organoid

Get tips on using mTeSR™1 to perform Stem cell culture media Human WA09 hESC

Get tips on using Cell Death Detection ELISA to perform Apoptosis assay cell type - Human endometrial stromal cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Human Seminal vesicles

Get tips on using ChIP Kit Magnetic - One Step (ab156907) to perform ChIP Human - MIA PaCa-2

Get tips on using ChIP Kit Magnetic - One Step (ab156907) to perform ChIP Human - MDA-MB-231

Get tips on using siRNA Wee1 to perform siRNA / miRNA gene silencing Human - SKOV-3 Wee-1

Get tips on using siRNA RBM3 to perform siRNA / miRNA gene silencing Human - MIA PaCa-2 RBM3

Get tips on using siRNA RIP3 to perform siRNA / miRNA gene silencing Human - MIA PaCa-2 RIP3