Immunohistochemistry Alpha smooth muscle Actin Mouse -NA-

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using hCas9 to perform CRISPR Rat - Activation CD2

Get tips on using MLM3636 to perform CRISPR Human - Activation VEGFA

Get tips on using Cas9m4-VP64 to perform CRISPR Human - Activation NEUROD1

Get tips on using phREX1-Luc to perform CRISPR Human - Activation REX1

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Activation α-synuclein

Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation ASCL1

Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation RhoGDIα

Get tips on using M-ST1n-VP64 to perform CRISPR Human - Activation RHOXF2

Get tips on using M-ST1n-VP64 to perform CRISPR Human - Activation MIAT