RNA isolation / purification Tissue Mouse

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - Mouse lung tissue

Get tips on using Mouse KIM-1 ELISA Kit to perform ELISA Mouse - KIM-1

Get tips on using Mouse IGF1 ELISA Kit (ab100695) to perform ELISA Mouse - IGF-I

Get tips on using GM-CSF Mouse ELISA Kit to perform ELISA Mouse - GM-CSF

Get tips on using Mouse GM-CSF DuoSet ELISA to perform ELISA Mouse - GM-CSF

Get tips on using Mouse Dkk-1 ELISA Kit to perform ELISA Mouse - Dkk-1

Get tips on using Mouse BMP2 ELISA Kit (ab119582) to perform ELISA Mouse - BMP-2

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.