rna-isolation-purification-tissue-rat-spinal-cord

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized BEAS-2B

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized A2780/DDP

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human keratinocytes

Get tips on using Alexa Fluor® 647 Rat Anti-Mouse CD206 to perform Flow cytometry Anti-bodies Mouse - CD206

Get tips on using PE-Cy™7 Rat Anti-Mouse CD86 to perform Flow cytometry Anti-bodies Mouse - CD86

Get tips on using Alexa Fluor® 700 Rat Anti-Mouse CD86 to perform Flow cytometry Anti-bodies Mouse - CD86

Get tips on using PerCP-Cy™5.5 Rat Anti-Mouse CD19 to perform Flow cytometry Anti-bodies Mouse - CD19

Get tips on using PerCP-Cy™5.5 Rat Anti-Mouse CD8a to perform Flow cytometry Anti-bodies Mouse - CD8a

Get tips on using PerCP-Cy™5.5 Rat Anti-Mouse CD4 to perform Flow cytometry Anti-bodies Mouse - CD4