CRISPR Hamster Deletion CHO-K1

Get tips on using gRNA_Cloning Vector to perform CRISPR Mouse - Deletion RMA cells Trh4

Get tips on using CAG-Cas9 to perform CRISPR Mouse - Deletion Neuro 2a Rab38

Get tips on using lentiCas9-Blast to perform CRISPR Mouse - Deletion RAW 264.7 Casp1

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Deletion 3T3-L1 TEAD

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Deletion MGAT1

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Deletion ATM

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Deletion APC

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Deletion HPRT1_A

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Deletion OCLN

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.