ChIP H3K36Me3 Bovine Rabbit

Get tips on using Cyclin D1 Recombinant Rabbit Monoclonal Antibody (SP4) to perform Western blotting Cyclin D1

Get tips on using Anti-β2-Microglobulin antibody produced in rabbit to perform Western blotting β₂ microglobulin

Get tips on using Anti-AP-1 antibody produced in rabbit to perform Western blotting C-Jun

Get tips on using LC3B (D11) XP® Rabbit mAb (Biotinylated) to perform Autophagy assay cell type - GL261

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb to perform Autophagy assay cell type - GL15

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb to perform Autophagy assay cell type - U937

Get tips on using Anti-p62/SQSTM1 antibody produced in rabbit to perform Autophagy assay cell type - HeLa

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb to perform Autophagy assay cell type - A549

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.