Immunohistochemistry Wilms Tumor 1 (WT1) Rabbit Mouse

Get tips on using Atg16L1 (D6D5) Rabbit mAb to perform Autophagy assay cell type - HEK 293

Get tips on using mTOR (7C10) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Atg5 (D5G3) Rabbit mAb to perform Autophagy assay cell type - HLE-B3

Get tips on using Mouse/Rat CD34 Antibody to perform Immunohistochemistry Mouse - CD34

Get tips on using Mouse/Rat TrkC Antibody to perform Immunohistochemistry Mouse - TrkC

Get tips on using Atg12 (D88H11) Rabbit mAb to perform Autophagy assay cell type - RPE-J cells

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - Hippocampal neural stem cells