RNA isolation / purification Tissue

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Is a bacterial nuclease contamination possible during protein purification? Discussion

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Discussions Is a bacterial nuclease contamination possible during protein purification?

Protein expression and purification Yeast - Pichia pastoris hmPRα Experiment

Proteins Protein expression and purification Yeast Pichia pastoris hmPRα

Protein expression and purification Yeast - Pichia pastoris Chymase Experiment

Proteins Protein expression and purification Yeast Pichia pastoris Chymase

Protein expression and purification Yeast - Saccharomyces cerevisiae ΔHSPA5 Experiment

Proteins Protein expression and purification Yeast Saccharomyces cerevisiae ΔHSPA5

Protein expression and purification Insect cells - Sf9 Drosha Experiment

Proteins Protein expression and purification Insect cells Sf9 Drosha

Protein expression and purification Insect cells - Hi5 TYR Experiment

Proteins Protein expression and purification Insect cells Hi5 TYR

Protein expression and purification Insect cells - S2 HER2 Experiment

Proteins Protein expression and purification Insect cells S2 HER2

Protein expression and purification Mammalian cells - HeLa ChaC1 Experiment

Proteins Protein expression and purification Mammalian cells HeLa ChaC1

Protein expression and purification Bacteria - Escherichia coli medin Experiment

Proteins Protein expression and purification Bacteria Escherichia coli medin

Protein expression and purification Bacteria - Escherichia coli IFNA2 Experiment

Proteins Protein expression and purification Bacteria Escherichia coli IFNA2

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