siRNA / miRNA gene silencing Human HCT-116

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using HES1 (D6P2U) Rabbit mAb #11988 to perform Immunohistochemistry Human - Hes1

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray RNA amplification & Labeling - Human endometrial stromal cells Biotin

Get tips on using PR Antibody (F-4): sc-166169 to perform Immunohistochemistry Human - PR

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform RNA amplification & labeling Mammalian - RNA, Human Endometrial Stromal cells Biotin

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Human - Endometrial stromal cells Target preparation kit (Amplification + Hybridization + control)

Get tips on using Sox-9 Antibody (E-9): sc-166505 to perform Immunohistochemistry Human - SOX9

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Kidney