RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized SKBR3, MDA-MB231 and MCF7

Get tips on using Absolutely RNA FFPE Kit to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using NucleoSpin® RNA/Protein to perform RNA isolation / purification Tissue - Rat Hippocampus

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.