Cell Culture Contamination Detection Kit

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human marrow stromal cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human bronchial epithelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human aortic endothelial cells

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary canine marrow stromal cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary canine marrow stromal cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary canine aortic endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human endometrial stromal cells

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human endometrial stromal cells

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat osteoblasts