Protein isolation Mammalian cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform DNA isolation / purification Tissue - kidney

Get tips on using Maj-NPLP to perform Protein Expression Prokaryotic cells - E. coli M. japonicus neuroparsin-like peptide

Get tips on using pET-PvD1 to perform Protein Expression Prokaryotic cells - E. coli PvD1, from Phaseolus vulgaris L.

Get tips on using pTip-QC2-gi_21221697 to perform Protein Expression Prokaryotic cells - R. erythropolis putative gntR-family regulator

Get tips on using pSS1-EBA140RIII–V to perform Protein Expression Prokaryotic cells - L. lactis EBA140RIII–V P. falciparum

Get tips on using pPICZαA‐opt‐RABV‐G to perform Protein Expression Eukaryotic cells - P. pastoris opt‐RABV‐G

Get tips on using pET-28a(+)-g-α13 to perform Protein Expression Prokaryotic cells - E. coli α-13 giardin

Get tips on using pET20b-chIL-7/H to perform Protein Expression Prokaryotic cells - E. coli chicken IL-7

Get tips on using Corning™ Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix to perform Mammalian cell culture media HSG cells