crispr-mouse-deletion-raw-264-7-dcstamp

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized DG-75

Get tips on using ChIP Kit Magnetic - One Step (ab156907) to perform ChIP Human - SMMC-7721

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized ZR-75-1

Get tips on using Annexin V-FITC Apoptosis Kit to perform Apoptosis assay cell type - SMMC-7721, HEPG2

Get tips on using Annexin V-FITC Apoptosis Kit to perform Apoptosis assay cell type - SMMC-7721, HEPG2

Get tips on using Polybrene Infection / Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines SMMC-7721

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized ZR-75-1

Get tips on using Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Point mutation 786-O REGγ

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines SMMC-7721