siRNA / RNAi /miRNA transfection Human Cells A549

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

Get tips on using Tau Protein Ladder, 6 isoforms human to perform Protein Ladder Immunofluorescence

Get tips on using Human Notch-1 Intracellular Domain Antibody to perform Western blotting Notch1

Get tips on using Active BDNF (Human, Rat) ELISA Kit to perform ELISA Mouse - GDNF

Get tips on using Human TGF Beta 1 PicoKine™ ELISA Kit to perform ELISA Human - TGF-beta 1

Get tips on using Human IL-1 Beta PicoKine™ ELISA Kit to perform ELISA Human - IL-1 beta

Get tips on using Human Total ER alpha/NR3A1 DuoSet IC ELISA to perform ELISA Human - Estrogen receptor (ESRs)

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human peripheral blood mononuclear cells