DNA isolation / purification Cells Immortalized cell lines

Get tips on using EasySep™ HLA T Cell Enrichment Kit to perform Cell Isolation HLA T Cell

Get tips on using RosetteSep™ HLA T Cell Enrichment Cocktail to perform Cell Isolation HLA T Cell

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Fluorescent High Range DNA Ladder to perform DNA Ladder Long Range

Get tips on using Low Range DNA Ruler Plus to perform DNA Ladder Low Range

Get tips on using Low Molecular Weight DNA Ladder to perform DNA Ladder Low Range

Get tips on using Fluorescent Low Range DNA Ladder to perform DNA Ladder Low Range

Get tips on using Fluorescent Long Range DNA Ladder to perform DNA Ladder Long Range

Get tips on using Fluorescent Mid Range DNA Ladder to perform DNA Ladder Medium Range

Get tips on using Fluorescent 20 bp DNA Ladder to perform DNA Ladder 20 bp