When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using Tau Protein Ladder, 6 isoforms human to perform Protein Ladder Immunofluorescence
Get tips on using RosetteSep™ Human Monocyte Enrichment Cocktail to perform Cell Isolation Monocyte
Get tips on using EasySep™ Human Monocyte Enrichment Kit to perform Cell Isolation Monocyte
Get tips on using EasySep™ Human Monocyte Isolation Kit to perform Cell Isolation Monocyte
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using Dynabeads™ Untouched™ Human T Cells Kit to perform Cell Isolation Human T cells
Get tips on using EasySep™ Direct Human T Cell Isolation Kit to perform Cell Isolation Human T cells
Get tips on using Human TGF Beta 1 PicoKine™ ELISA Kit to perform ELISA Human - TGF-beta 1
Get tips on using Human IL-1 Beta PicoKine™ ELISA Kit to perform ELISA Human - IL-1 beta
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