Get tips on using Platinum™ Taq DNA Polymerase to perform PCR Hot start PCR - Bacterial DNA
Get tips on using EZ DNA Methylation-Direct Kit to perform PCR Methylation specific PCR - Mammalian DNA
Get tips on using EZ DNA Methylation-Gold Kit to perform PCR Methylation specific PCR - Mammalian DNA
Get tips on using Hot Start Taq DNA Polymerase to perform PCR Hot start PCR - Mammalian DNA
Get tips on using Platinum™ Taq DNA Polymerase to perform PCR Hot start PCR - Mammalian DNA
Get tips on using FastStart™ Taq DNA Polymerase to perform PCR Conventional / Qualitative PCR - mammalian DNA
Get tips on using Platinum™ Taq DNA Polymerase to perform PCR Conventional / Qualitative PCR - mammalian DNA
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The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
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