rna-isolation-purification-tissue-mouse-spleen

Get tips on using PE anti-mouse CD19 Antibody to perform Flow cytometry Anti-bodies Mouse - CD19

Get tips on using Purified anti-mouse CD19 Antibody to perform Flow cytometry Anti-bodies Mouse - CD19

Get tips on using Purified anti-mouse CD11c Antibody to perform Flow cytometry Anti-bodies Mouse - CD11c

Get tips on using APC anti-mouse CD45 Antibody to perform Flow cytometry Anti-bodies Mouse - CD45

Get tips on using Mouse/Rat Leptin Quantikine ELISA Kit to perform ELISA Mouse - Leptin

Get tips on using Mouse TNFSF11/RANKL PicoKine™ ELISA Kit to perform ELISA Mouse - RANK L

Get tips on using PE anti-mouse CD146 Antibody to perform Flow cytometry Anti-bodies Mouse - CD146/MCAM

Get tips on using InVivoMAb anti-mouse CD16/CD32 to perform Flow cytometry Anti-bodies Mouse - CD16/CD32

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.