rna-isolation-purification-cells-primary-canine-peripheral-blood-mononuclear-cells

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Tissue - rat pancreas tissue

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Rat Trachea

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Rat Thymus

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Rat Skin

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Rat Retina

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Rat Hippocampus

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Rat Esophagus

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Rat Cerebellum

Get tips on using RNeasy Plus Micro Kit to perform RNA isolation / purification Tissue - Rat Adipose

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.