Get tips on using Ovation® RNA Amplification System V2 to perform Microarray RNA amplification & Labeling - Mouse cochlaea Biotin
Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Primary cells Rat aortic smooth muscle cells (rASMC)
Get tips on using Mouse Prolactin DuoSet ELISA to perform ELISA Mouse - PRL
Get tips on using Mouse Myeloperoxidase DuoSet ELISA to perform ELISA Mouse - MPO
Get tips on using Mouse Leptin ELISA Kit to perform ELISA Mouse - Leptin
Get tips on using Mouse Decorin ELISA Kit to perform ELISA Mouse - Decorin
Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human lung fibroblasts (HLF)
Get tips on using Mouse Osteopontin/OPN Antibody to perform Immunohistochemistry Mouse - Spp1/OPN
Get tips on using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® to perform RNA sequencing Mouse - Neuro 2a
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
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