rna-isolation-purification-cells-primary-mouse-ventricles

Get tips on using Mouse MCP1 ELISA Kit (ab100721) to perform ELISA Mouse - MCP1

Get tips on using Mouse proMMP9 ELISA Kit (ab100732) to perform ELISA Mouse - MMP9

Get tips on using Mouse Leptin ELISA Kit (ab100718) to perform ELISA Mouse - Leptin

Get tips on using Mouse GDNF ELISA Kit (ab171178) to perform ELISA Mouse - GDNF

Get tips on using Mouse Fibronectin ELISA Kit (ab108849) to perform ELISA Mouse - Fibronectin

Get tips on using Human/Mouse BDNF DuoSet ELISA to perform ELISA Mouse - BDNF

Get tips on using Mouse Adiponectin/Acrp30 DuoSet ELISA to perform ELISA Mouse - Adiponectin

Get tips on using Mouse Adiponectin ELISA Kit (ab108785) to perform ELISA Mouse - Adiponectin

Get tips on using Atg12 Antibody (Mouse Specific) #2011 to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.