Site Directed Mutagenesis (SDM) Human Point mutation THP-1

Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - THP-1

Get tips on using PE anti-human CD111 (Nectin-1) Antibody to perform Flow cytometry Anti-bodies Human - CD111/Nectin-1

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Human Chitinase 3-like 1 DuoSet ELISA to perform ELISA Human - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

Get tips on using Human Notch-1 Intracellular Domain Antibody to perform Western blotting Notch1

Get tips on using Human KIM1 / TIM-1 PicoKine™ ELISA Kit to perform ELISA Human - KIM-1

Get tips on using Human Total HO-1/HMOX1 DuoSet IC ELISA to perform ELISA Human - HO-1

Get tips on using Human TGF Beta 1 PicoKine™ ELISA Kit to perform ELISA Human - TGF-beta 1