rna-isolation-purification-tissue-human-mouth

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel

Get tips on using EZ1 RNA Tissue Mini Kit (48) to perform RNA isolation / purification Tissue - Human FFPE tissue

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - human brain tissue

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - human liver tissue

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - human breast tissue

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - human kidney tissue

Get tips on using PureLink™ FFPE RNA Isolation Kit to perform RNA isolation / purification Tissue - human kidney tissue

Get tips on using InviTrap Spin Tissue RNA Mini Kit to perform RNA isolation / purification Tissue - Human Artery / Aorta