shRNA gene silencing Human Neuroblastoma cells (SH-SY5Y)

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using FuGENE® HD Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human pulmonary artery smooth muscle cells (HPASMC)

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human pulmonary artery smooth muscle cells (HPASMC)

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human pulmonary arterial smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical vein smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical artery smooth muscle cells