ChIP acH3 Rat Sheep BEF Tag

Get tips on using RAB8A siRNA to perform siRNA / miRNA gene silencing Human - HEK293 Rab8a

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

Get tips on using High Range DNA Ladder to perform DNA Ladder Long Range

Get tips on using Low Range DNA Ladder to perform DNA Ladder Low Range

Get tips on using Mid Range DNA Ladder to perform DNA Ladder Medium Range

Get tips on using Wide-Range DNA Ladder to perform DNA Ladder Long Range

Get tips on using Medium Range DNA Ruler to perform DNA Ladder Medium Range

Get tips on using Rac1/2/3 Antibody #2465 to perform Western blotting Rac1

Get tips on using Fluorescent High Range DNA Ladder to perform DNA Ladder Long Range

Get tips on using Low Range DNA Ruler Plus to perform DNA Ladder Low Range