The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.
Get tips on using Fenozol to perform RNA isolation / purification Tissue - human aorta tissue
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human breast tissue
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human lung tissue
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human placental tissue
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human brain tissue
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human aorta tissue
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat brain tissue
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - rat brain tissue
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat spleen tissue
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