rna-isolation-purification-tissue-mouse-mammary-glands

Get tips on using PE Rat Anti-Mouse Ly-6G to perform Flow cytometry Anti-bodies Mouse - Ly6C/Gr-1/Ly6G

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - RAW264.7

Get tips on using Mouse Sonic Hedgehog/Shh DuoSet ELISA to perform ELISA Mouse - ShhN

Get tips on using Mouse MPO ELISA Kit (Myeloperoxidase) (ab155458) to perform ELISA Mouse - MPO

Get tips on using pro-MMP-9 Mouse ELISA Kit to perform ELISA Mouse - MMP9

Get tips on using Mouse GDNF PicoKine™ ELISA Kit to perform ELISA Mouse - GDNF

Get tips on using Mouse Fibronectin PicoKine™ ELISA Kit to perform ELISA Mouse - Fibronectin

Get tips on using Mouse Decorin ELISA Kit (DCN) (ab155454) to perform ELISA Mouse - Decorin

Get tips on using Mouse BDNF PicoKine™ ELISA Kit to perform ELISA Mouse - BDNF

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.