siRNA / RNAi /miRNA transfection Human Cells Jurkat cells

Get tips on using Dual-Luciferase® Reporter Assay System to perform Reporter gene assay luciferase - primary human endometrial stromal cells

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - human primary corneal epithelial cells

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary rat lymph node cells

Get tips on using Ovation® RNA Amplification System V2 to perform Microarray RNA amplification & Labeling - Human endometrial stromal cells Cyanine 3-pCp

Get tips on using Quant-iT™ RiboGreen™ RNA Assay Kit to perform RNA quantification Fuorimetric - human peripheral blood mononuclear cells (PBMCs)

Get tips on using RediPlate™ 96 RiboGreen™ RNA Quantitation Kit to perform RNA quantification Fuorimetric - human colorectal adenocarcinoma cells (CL-187)

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - HeLa cells human cervical cancer

Get tips on using Neural Progenitor Medium 2 to perform Stem cell Differentiation media Differentiation of Human PSC into Neural progenitor cells