siRNA / miRNA gene silencing Human Caki-2

Get tips on using TIMP2 (D18B7) Rabbit mAb #5738 to perform Western blotting TIMP-2

Get tips on using Anti-TIMP2 antibody [3A4] (ab1828) to perform Western blotting TIMP-2

Get tips on using Mouse BMP2 ELISA Kit (ab119582) to perform ELISA Mouse - BMP-2

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using GeneArt™ Site-Directed Mutagenesis PLUS System to perform Site Directed Mutagenesis (SDM) Rat - Point mutation Rat-2 PIK3CB

Get tips on using pVRB2B3 to perform Protein Expression Eukaryotic cells - HEK293 hβ-defensin 2/3

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized C3H-10T1/2

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized U-2 OS

Get tips on using Fluoro hROS to perform ROS assay cell type - MiaPaCa-2 pancreatic carcinoma