CRISPR Mouse Deletion ES (embryonic stem) cells

Get tips on using Estrogen Receptor alpha siRNA (m) to perform siRNA / miRNA gene silencing Mouse - mESC ERα

Get tips on using RNeasy Fibrous Tissue Mini Kit to perform RNA isolation / purification Tissue - Mouse Esophagus

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Stomach

Get tips on using StemSep™ Human CD34 Positive Selection Cocktail to perform Cell Isolation CD34+ cells

Get tips on using Recombinant Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063) to perform Immunohistochemistry Mouse - ERα