rna-isolation-purification-tissue-mouse-lung

Get tips on using Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit to perform ELISA Mouse - RANK L

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Human/Mouse/Rat SOX2 Antibody to perform Western blotting SOX2

Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - LTEP-a-2 lung adenocarcenoma

Get tips on using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® to perform RNA sequencing Mouse - Neuro 2a

Get tips on using FoxP3 Antibody, anti-mouse, PE, REAfinity™ to perform Flow cytometry Anti-bodies Mouse - FOXP3

Get tips on using Ion AmpliSeq™ Cancer Hotspot Panel v2 to perform Cell line authentication Lung carcinoma A549 cell line

Get tips on using DMEM:F12 with HEPES, Glucose and L-Glutamine to perform 3D Cell Culture Media Human lung cancer organoids

Get tips on using Gibco™DMEM/F-12, no glutamine to perform 3D Cell Culture Media hiPSC-derived lung organoids

Get tips on using Wizard® Plus SV Minipreps DNA Purification System Technical Bulletin to perform Plasmid Isolation Streptomyces spp