siRNA / miRNA gene silencing Human EM-2

Get tips on using Cellular DNA Fragmentation ELISA to perform DNA Damage Assay Saos-2

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay Saos-2

Get tips on using Annexin V-FITC Apoptosis Kit to perform Necrosis MIA PaCa-2

Get tips on using Apoptosis and Necrosis Quantification Kit to perform Necrosis MIA PaCa-2

Get tips on using TIMP2 (D18B7) Rabbit mAb #5738 to perform Western blotting TIMP-2

Get tips on using Anti-TIMP2 antibody [3A4] (ab1828) to perform Western blotting TIMP-2

Get tips on using Mouse BMP2 ELISA Kit (ab119582) to perform ELISA Mouse - BMP-2

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using GeneArt™ Site-Directed Mutagenesis PLUS System to perform Site Directed Mutagenesis (SDM) Rat - Point mutation Rat-2 PIK3CB