siRNA / miRNA gene silencing Mouse MLO‐Y4

Get tips on using CD4 Monoclonal Antibody (GK1.5), eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD4

Get tips on using CD34 Monoclonal Antibody (RAM34), eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD34

Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell culture media Mouse pericytes

Get tips on using CD133 (Prominin-1) Monoclonal Antibody (13A4), APC, eBioscience™ to perform Immunohistochemistry Mouse - CD133

Get tips on using Gibco™Ham's F-10 Nutrient Mix to perform Stem cell culture media Mouse myoblasts cells

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using pET-21b(+)-mTNFα to perform Protein Expression Prokaryotic cells - E. coli mouse TNF-(α)

Get tips on using EmbryoMax MEF Conditioned Media to perform Stem cell culture media Mouse trophoblast stem cells

Get tips on using ATG5 Antibody (N-term) to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)