Site Directed Mutagenesis (SDM) Human Point mutation Caco-2

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Human - MDA-MB-231

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - HEK 293

Get tips on using pET-28b-hBCO1 to perform Protein Expression Prokaryotic cells - E. coli human BCO1

Get tips on using Keratinocyte SFM (1X) to perform 3D Cell Culture Media Human prostate organoid

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Repression HPV-18 E6

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Repression HPV-18 E7

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human pulmonary artery smooth muscle cells (HPASMC)

Get tips on using mTeSR™1 to perform Stem cell culture media Human WA09 hESC

Get tips on using psiCHECK-2vector to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells