siRNA / RNAi /miRNA transfection Human Cells Cal 27 cells

A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Ovation® RNA Amplification System V2 to perform Microarray RNA amplification & Labeling - Human endometrial stromal cells Cyanine 3-pCp

Get tips on using Quant-iT™ RiboGreen™ RNA Assay Kit to perform RNA quantification Fuorimetric - human peripheral blood mononuclear cells (PBMCs)

Get tips on using Neural Progenitor Medium 2 to perform Stem cell Differentiation media Differentiation of Human PSC into Neural progenitor cells

Get tips on using Gibco™ MEM α, Nucleosides to perform Stem cell Differentiation media Human oogonial stem cells differentiation into oocytes

Get tips on using DeadEnd™ Fluorometric TUNEL System to perform TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells

Get tips on using TUNEL Assay Kit - BrdU-Red to perform TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells

Get tips on using Gibco™Essential 8™ Medium to perform Stem cell Differentiation media hiPSC differentiation into Human Neuronal cells

Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - U2OS (human bone osteosarcoma epithelial cells)